Clinical study of telbivudine on PCR technique to detect the DNA of Mycobacterium tuberculosis
10.3760/cma.j.issn.1008-6706.2011.s2.005
- VernacularTitle:聚合酶链反应检测结核杆菌DNA的临床研究
- Author:
Ping HUANG
;
Long LIN
;
Yingxia DENG
;
Ying LIN
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Polymerase chain reaction;
Colony count,microbial;
Fluorescent antibody technique;
Tuberculosis
- From:
Chinese Journal of Primary Medicine and Pharmacy
2011;18(z2):10-11
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo value fluorescent quantified PCR (polymerase chain reaction) technique to detect the DNA of Mycobacterium tuberculosis from clinical patients.MethodsDetect the DNA of TB from the sputum of 80 active tuberculosis and hydrothorax of 40 tuberculous pleuritis.At equal pace,make a comparison to proceed microscopic examination and cultivation.ResultsWith fluorescent quantified PCR,56.2％ (45/80),27.5％ (11/40)positive detection from active tuberculosis and tuberculous pleuritis respectively.16.2％ ( 13/80 ),0.0％ positive specimen were detected with microscopic examination in two group of patients.Cultivation got 37.5％ (30/80),2.5％(1/40) positive detection respectively.ConclusionFluorescent quantified PCR was more sensitive and specific than traditional methods,and valuable in the microscopic examination and cultivation negative patients especially.
