Inhibitory effects of DNT cells on growth of pancreatic cancer
10.3760/cma.j.issn.1673.4203.2012.02.008
- VernacularTitle:DNT细胞对体内外胰腺癌生长的抑制作用
- Author:
Yin LU
;
Jiong CHEN
;
Min DU
;
Renbao YANG
;
Yunlian XIA
;
Chunsheng ZHENG
;
Hangcheng ZHOU
;
Wen WU
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Malignant;
CD4-CD8- DNTcell;
Immunologic;
Depression
- From:
International Journal of Surgery
2012;39(2):97-100
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo find out the inhibitory effects of CD4 - CD8 - DNT cells on growth of which depresses the pancreatic cancer in vitro and in vivo.Methods The inhibitory effects of DNT cells on the growth of Panc- 1 were studied in vitro by MTT method.Eighteen BALB/c mice were divided into 3 groups randomly.Human pancreatic cancer xenografts were established in 2 groups randomly.The last group was injected the cell suspension which comprises DNT and Panc- 1 cells ( Panc- 1∶ DNT =1∶ 5 ).When the diameter of tumor was about 5 mm,the first 2 group mice were further divided into 2 groups randomly.One was control,treated with distilled water.The other was treated with celebrex (4 mg/d).The size of the tumors was calculated every 2 weeks and tumor growth curve was depicted.At the end of the treatments,the mice were sacrific and the tumors were harvested.The tumor inhibition rate was calculated.Results( 1 ) MTT study showed that DNT cells produced a dose- dependent inhibition of Panc- 1 proliferation in vitro.(2) The growth of transplanted pancreatic cancer was down-regulated by treatment of DNT cells.ConclusionDNT cells can inhibit the growth of pancreatic cancer in vitro and in vivo.
