Experimental study of Gexia-Zhuyu decoction combined with cyclophosphamide on tumor interstitial in rats of EAC liver cancer
10.3760/cma.j.issn.1673-4246.2012.01.009
- VernacularTitle:膈下逐瘀汤联合环磷酰胺对EAC肝癌小鼠肿瘤间质血管生成的影响
- Author:
Hongxia ZHAO
;
Ying WANG
;
Jinru ZHAO
;
Shanli WANG
;
Binbin LI
;
Wei SONG
;
Chunying LIU
- Publication Type:Journal Article
- Keywords:
Gexia-Zhuyu decoction;
Cyclophosphamide;
Liver cancer
- From:
International Journal of Traditional Chinese Medicine
2012;34(1):25-27
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the anti-cancer effects and mechanism of Gexia-Zhuyu decoction combined with cyclophosphamide on EAC liver cancer mice.MethodsChoose EAC liver cancer mice cell lines and production of animal models of tumor.The mice were completely randomized into blank group,model group,the Chinese medicine group,CTX group,Chinese medicine and CTX group.The blank group and model group were given normal saline 0.4 ml/d; traditional Chinese medicine group was administrated with Gexia-Zhuyu decoction (11 g/kg body weight ) 0.4 ml/d gavage; CTX group was given intraperitoneal injection of 2 mg/ml of cyclophosphamide 0.4 ml/d; and traditional Chinese medicine and CTX group was given Gexia Zhuyu decoction 0.4 ml/d orally and CTX intraperitoneal injection of 0.4 ml/d.Ten days after the administration,the body weight of mice and the general condition were tested,and morphology was observed under the light microscope electron microscope mesenchymal tumor angiogenesis situation.Results Gexia-Zhuyu decoction combined with cyclophosphamide in mice could improve appetite,gain weight,and greatly reduce the tumor stroma in the number of capillaries under the electron microscope together with significant pathological morphological changes.Conclusion Gexia-Zhuyu decoction combined with cyclophosphamide had anti-cancer effects which can improve the quality of life and inhibit the growth of tumor in interstitial capillaries.
