Effect of irrigation of peritoneal cavity with warm normal saline on expression of transforming growth factor beta-1 in the peritoneum during the laparoscopic operation
10.3760/cma.j.issn.1673-4203.2012.01.009
- VernacularTitle:腹腔镜手术中温热生理盐水腹腔冲洗对腹膜组织TGFβ1表达的影响
- Author:
Fei CHEN
;
Quangen GAO
;
Zeqiang REN
;
Xiangnong LI
- Publication Type:Journal Article
- Keywords:
Transforming growth factor;
Laparoscopy;
Gastroduodenal ulcer perforation;
Warm normal saline
- From:
International Journal of Surgery
2012;39(1):23-26
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effects of the temperature of saline for irrigation of peritoneal cavity on expression of transforming growth factor beta- 1 in the peritoneum.MethodsSixty patients scheduled for the laparoscopic repair of gastroduodenal benign ulcer perforation were randomized into two groups then surgically treated with various temperature saline.Peritoneal biopsies were taken at the beginning and end of surgery.Tissue concentrations of total and active were measured using enzymelinked immunosorbent assa technique.ResultsAt the start of surgery,there were no significant differences between groups in the total and active fractions of TGFβ1 (P ＞0.05).At the end of procedure,the peritoneal total( 135.8 ±52.8) pg/mL and active( 136.5 ± 33.0) pg/mL concentrations were significantly lower(P ＜ 0.05) in patients receiving warm normal saline.A light,nonsignificant increase in total(361.3 ± 178.9) pg/mL and active ( 198.3 ± 87.5) pg/mL TGFβ1 levels was observed in patients receiving cold saline(P ＞ 0.05).ConclusionsThe choice of warm normal saline used in irrigation of peritoneal cavity during the laparoscopic operation can decrease peritoneal transforming growth factor beta-1 concentrations.Because of the broad biological effects of TGF31,including regulation of peritoneal healing and oncological processes,this observation may have important clinical perspective.
