A study of downregulation of miR-93 on the suppression of human glioma cell growth and invasion

VernacularTitle:下调miR-93表达抑制人脑胶质瘤细胞生长和侵袭的研究
Author: Anling ZHANG ; Kun WANG ; Guangxiu WANG ; Zhifan JIA ; Peiyu PU
Publication Type:Journal Article
Keywords: Brain neoplasms; Glioma; Oligonucleotides,antisense
From: Journal of International Oncology 2012;39(1):68-72
CountryChina
Language:Chinese
Abstract: ObjectiveTo confirm the effect of miR-93 inhibitor in glioma cell growth and invasion.MethodsMalignant glioma cells were transfected with miR-93 inhibitor by lipofectamin to downregulate their overexpression of miR-93.Real time-PCR was taken to measure miR-93 expression after transfection.The cell cycle kinetics and cell growth rate were detected by flowcytometry and MTT assay,the cell proliferative ability was evaluated by soft agar assay,and the invasive ability was detected by transwell assay.ResultsThe highlevel expression of miR-93 was downregulated effectively in glioma cells after transfecting the miR-93 inhibitor.Meanwhile,the cell cycle progress was delayed,S phase cells were reduced,the speed of growth was slowed,cloning formation ability was receded,the number of cells through the matrigel was reduced,and invasive ability was significantly repressed.ConclusionDownregulation of miR-93 expression could inhibit the proliferative ability and invasive ability of glioma cells.