Quantitative Determination of Bakkenolide D in Petasites japonicus and Farfugium japonicum by HPLC/UV.
10.20307/nps.2017.23.4.270
- Author:
Norman G QUILANTANG
1
;
Ki Ho LEE
;
Dong Gu LEE
;
Ju Sung LEE
;
EunJu CHO
;
Hyun Young KIM
;
Sanghyun LEE
Author Information
1. Department of Integrative Plant Science, Chung-Ang University, Anseong 17546, Korea. slee@cau.ac.kr
- Publication Type:Original Article
- Keywords:
Bakkenolide D;
Petasites japonicus;
Farfugium japonicum;
HPLC/UV
- MeSH:
Chromatography, High Pressure Liquid;
Dietary Supplements;
Petasites*
- From:Natural Product Sciences
2017;23(4):270-273
- CountryRepublic of Korea
- Language:English
-
Abstract:
A quantitative analysis of bakkenolide D in the different parts of Petasites japonicus and Farfugium japonicum was performed by HPLC. A gradient HPLC elution system with a mobile phase consisting of water:acetonitrile solution (20:80 to 0:100 for 45 min) was followed and an INNO C₁₈ column was used for the chromatographic separation. The injection volume, flow rate, and UV detection were 10 µL, 1 mL/min, and 290 nm, respectively. Results show that both species showed the highest amount of bakkenolide D in the roots being 107.203 and 166.103 mg/g for P. japonicas and F. japonicum, respectively. Content analysis on the different parts of both plants displayed remarkably lower values which ranged from 0.403 – 4.419 and 7.252 – 32.614 mg/g for P. japonicas and F. japonicum, respectively. The results show that the roots of both plants are rich in bakkenolide D showing a promising use in the development of nutraceuticals and industrial application of the compound.
