Phenolic Compounds from the Leaves of Homonoia riparia and their Inhibitory Effects on Advanced Glycation End Product Formation.
10.20307/nps.2017.23.4.274
- Author:
Ik Soo LEE
1
;
Seung Hyun JUNG
;
Chan Sik KIM
;
Jin Sook KIM
Author Information
1. KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea. jskim@kiom.re.kr
- Publication Type:Original Article
- Keywords:
Homonoia riparia;
Euphorbiaceae;
Advanced glycation end product;
Zebrafish;
Diabetic complication
- MeSH:
Diabetes Complications;
Ethanol;
Euphorbiaceae;
Glucose;
In Vitro Techniques;
Natural Resources;
Phenol*;
Zebrafish
- From:Natural Product Sciences
2017;23(4):274-280
- CountryRepublic of Korea
- Language:English
-
Abstract:
In a search for novel treatments for diabetic complications from natural resources, we found that the ethyl acetate-soluble fraction from the 80% ethanol extract of the leaves of Homonoia riparia has a considerable inhibitory effect on advanced glycation end product (AGE) formation. Bioassay-guided isolation of this fraction resulted in identification of 15 phenolic compounds (1 – 15). These compounds were evaluated in vitro for inhibitory activity against the formation of AGE. The majority of tested compounds, excluding ethyl gallate (15), markedly inhibited AGE formation, with IC₅₀ values of 2.2 – 89.9 µM, compared with that of the positive control, aminoguanidine (IC₅₀ = 962.3 µM). In addition, the effects of active isolates on the dilation of hyaloid-retinal vessels induced by high glucose (HG) in larval zebrafish was investigated; (–)-epigallocatechin-3-O-gallate (6), corilagin (7), and desmanthine-2 (11) significantly decreased HG-induced dilation of hyaloid–retinal vessels compared with the HG-treated control group.
