The genetic association study of dendritic cell immunoreceptor polymorphisms in anti-CCP antibodynegative rheumatoid arthritis in Chinese Han population
10.3760/cma.j.issn.1007-7480.2012.01.002
- VernacularTitle:树突状细胞免疫受体基因多态性与抗环瓜氨酸肽抗体阴性类风湿关节炎相关性研究
- Author:
Jianping GUO
;
Xinyu WU
;
Xiaolan LU
;
Fangrui YIN
;
Xu LIU
;
Jing ZHAO
;
Yuan AN
;
Ru LI
;
Jing HE
;
Zhanguo LI
- Publication Type:Journal Article
- Keywords:
Arthrtis,rheumatoid;
Dendritic cells;
Genetic association;
Anti-cyclic citrullinated peptide antibody;
Chinese Han population
- From:
Chinese Journal of Rheumatology
2012;16(1):4-8
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThis work is aimed to investigate the possible association of dendritic cell immunoreceptor (DCIR) with rheumatoid arthritis (RA) susceptibility in Chinese Han population.Methods A total of 523 patients with RA and 510 healthy controls were genotyped for single-nucleotide polymorphism (SNP) rs2377422 and rs10840759.Association analyses were performed on the whole data set and on RA subsets based on the status of anti-cyclic citrullinated peptide antibody (CCP) in RA patients.Finally,we carried out the association analysis of rs2377422 with DCIR mRNA expression in RA patients.Statistical analysis used in this study included X2 test,Logistic regression,and Mann-Whitney U test.ResultsDCIR rs2377422 was found significantly associated with RA(allele analysis: OR 1.26; 95％CI 1.06～1.51,P=0.005; genotype analysis CC vs TT+TC: OR 1.34; 95％CI 1.18～2.06,P=0.004).Following stratification for anti-CCP antibody status,association of ra2377422 with anti-CCP-positive RA was observed(allele analysis: OR 1.22,95％CI 0.99～1.48,P=0.055).In contrast,the SNP rs2377422 was found specifically susceptible to anti-CCP-negative RA(allele analysis: OR 1.46; 95％CI 1.10～1.93,P=0.0091; genotype analysis CC vs TT+TC: OR 1.58;95％CI 1.01～2.47,P=0.043),despite loss of power in the analysis.DCIR gene transcription quantification analysis further proved the dominant effect of rs2480256 CC genotype on DCIR mRNA expression levels in RA patients (CC vs TT+TC: 0.429±0.069 vs 0.238±-0.023,U=1861,P=0.0015).ConclusionThe study provides evidence for the association between DCIR rs2377422 and RA,particularly with anti-CCP-negative RA in Chinese Han populations.
