An experimental study on the effects of membrane molecular weight cut off on a novel bioartificial liver system

VernacularTitle:膜截流分子量对新型生物人工肝支持系统内细胞材料影响的实验研究
Author: Xiaolei SHI ; Yue ZHANG ; Bing HAN ; Jinyang GU ; Xuehui CHU ; Jiangqiang XIAO ; Haozhen REN ; Jiajun TAN ; Yitao DIANG
Publication Type:Journal Article
Keywords: Bioartificial liver; Membrane molecular weight cut off; Hepatocytes
From: Chinese Journal of Hepatobiliary Surgery 2012;18(1):46-49
CountryChina
Language:Chinese
Abstract: ObjectiveTo study the effects of membrane molecular weight cut off on a novel bioartificial liver(BAL) system.MethodsHealthy beagles underwent 6-hour treatment with a BAL containing membrane with 200 kDa retention rating or 1200 kDa retention rating.The functional changes and cell viability were characterized.ResultsHepatocyte performance levels such as albumin secretion,urea synthesis and viability were significantly higher in the 200 kDa retention rating group when compared with the 1200 kDa retention rating group (P＜0.05).Significant levels of canine proteins were detected in the BAL medium from the 1200 kDa retention rating group.Fluorescence microscopy further verified that heavy deposition of canine IgG,IgM and complement (C3) on co-culture cells were obtained after BAL treatment in the 1200 kDa retention rating group.ConclusionsSmall membrane molecular weight cut off of BAL could reduce the transfer of xenoreactive antibodies into the BAL medium and improved the performance of the BAL.