Clinical values of urinary transforming growth factor-β1 macrophage inflammatory protein-1α and neutrophil gelatinase-associated hpocalin level in lupus nephritis
10.3760/cma.j.issn.1007-7480.2012.01.008
- VernacularTitle:狼疮肾炎尿液转化生长因子-β1巨噬细胞炎症蛋白-1α和中性粒细胞明胶酶相关脂质运载蛋白检测的临床意义
- Author:
Xinyue SHI
;
Hongbin LI
;
Zen XIAO
;
Duo ZHANG
;
Li GONG
- Publication Type:Journal Article
- Keywords:
Lupus nephritis;
Urinalysis;
Transforming growth factor beta;
Macrophage inflammatory protein-1;
Neutrophil gelatinase-associated lipocalin
- From:
Chinese Journal of Rheumatology
2012;16(1):33-37
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo determine whether there is correlation between the pathological classification and urinary level of transforming growth factor(TGF)-β1,macrophage inflammatory protein (MIP)-1α,and Neutrophil gelatinase-associated lipocalin (NGAL) of lupus nephritis.MethodsELISA was used to test the levels of urinary level of TGF-β1,MIP-lα and NGAL.The correlation between these three urinary markers and the pathological classifications,Austin score,histological semi-quantitative score and clinical data were analyzed.Comparisons between groups were performed with t-test,ANOVA and X2 test.Correlation analysis was performed with Pearson analysis.Results①There was significant difference in urinary TGF- β1,MIP1α,and NGAL levels when compared the lupus nephritis group,SLE patients without renal damage group and the normal control group [TGF-β1(351±219),(92±60),(74±29) pg/ml],[MIP-1α(18.0±15.5),(8.5±2.3),(7.1±1.9) pg/ml],[NGAL (1.104±0.519),(0.181±0.030),(0.146±0.024) ng/ml],while there was no significant difference between lupus nephritis (LN) group and patients with other glomerulonephritis.② There was significant difference in the three urinary markers when stratified the LN patients based on the pathological classifications,however,there was no significant difference between the LN group and other glomerulonephritis group.③ Analysis of the correlation between the three urinary markers and semiquantitative histopathological score of the LN patients showed that the urinary level of TGF-β1 and MsMI,GCI,TCI,VCI was closely related to each other,the urinary MIP-1α was related to and endol,and the dGAI and VCI was closely related, while the urinary NGAL was closely related with endol,dGAI and MsHI.The correlation analysis between the three urinary markers and acute and chronic pathological index score of the LN patients showed that TGF- β1 was correlated with the chronic index(r=0.89,P＜0.01 ),the MIP-lα and NGAL were significantly correlated with the activity index(r=0.71,P＜0.01 ; r=0.60,P＜0.01 ).Conclusion There is a positive correlation between the urine TGF-β1 level and chronic kidney disease.The urinary MIP-lαand NGAL are associated with active renal damage.
