Effect of resveratrol on the oxidative stress and antioxidant levels of different skeletal muscle mitochondrial subpopulations and insulin sensitivity in rats fed with high-fat diet
10.3760/cma.j.issn.1000-6699.2012.01.018
- VernacularTitle:白藜芦醇对高脂饮食大鼠骨骼肌不同线粒体亚群氧化、抗氧化水平和胰岛素敏感性的影响
- Author:
Lulu CHEN
;
Haohao ZHANG
;
Juan ZHENG
;
Xiang HU
;
Wen KONG
;
Di HU
;
Suxing WANG
- Publication Type:Journal Article
- Keywords:
Resveratrol;
Subsarcolemmal mitochondria;
Intermyofibrillar mitochondria;
Insulin resistance;
Oxidative stress
- From:
Chinese Journal of Endocrinology and Metabolism
2012;28(1):58-62
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of resveratrol on the oxidative stress and antioxidant levels of different mitochondrial subpopulations in the skeletal muscle and insulin sensitivity of rats fed with high-fat diet.MethodsMale SD rats,aged 8 weeks,were divided into normal chow (NC) group,high-fat diet(HF) group,high-fat diet plus resveratrol ( HFR ) group.After intervention for 8 weeks,the impacts of resveratrol on oxidative stress levels and antioxidant enzymes activities in subsarcolemmal ( SS ) and intermyofibrillar ( IMF ) mitochondria from skeletal muscle as well as general and skeletal mascle insulin sensitivity were assessed.ResultsCompared with NC group, insulin sensitivity was significantly reduced while reactiveoxygenspecies (ROS)and malondialdehyde(MDA) levels in SS and IMF mitochondria increased in HF group ( all P＜0.01 ).In addition,antioxidant enzyme activities were significantly decreased in SS mitochondrial and increased in IMF mitochondrial ( both P ＜ 0.05 ).Compared with HF group,the insulin sensitivity in HFR group was significantly improved.Moreover,the activities of antioxidant enzymes in SS and IMF mitochondrial were increased,and the oxidative stress levels returned to normal ( P ＜ 0.05 ).ConclusionResveratrol notably improves the oxidative stress of different skeletal muscle mitochondrial subpopulations and insulin resistance in rats fed with high-fat diet.
