Bone marrow mesenchymal stem cells combined with VEGF gene for the treatment of limb ischemia in rabbits
10.3760/cma.j.issn.1007-631X.2012.01.017
- VernacularTitle:骨髓间充质干细胞联合血管内皮生长因子基因治疗肢体缺血的实验研究
- Author:
Hai YUAN
;
Dianning DONG
;
Xing JIN
;
Yongxin ZOU
;
Xuejun WU
;
Xiangqian KONG
;
Jingyong ZHANG
;
Binbin GAO
;
Hua ZHOU
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Vascular endothelial growth factors;
Gene transfection;
Animal experimentation
- From:
Chinese Journal of General Surgery
2012;27(1):44-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate bone marrow mesenchymal stem cells combined with VEGF gene in the treatment of limb ischemia in rabbits.Methods The right hind limb ischemia model of New Zealand rabbit was established by superficial femoral artery excision and deep femoral artery ligation.Rabbits then were divided randomly into 4 groups: empty plasmid control group(EP group),bone marrow mesenchymal stem cells group(BMSC group),VEGF gene therapy group(VEGF group),combination bone mesenchymal stem cells and VEGF gene therapy group(BV group).There were 8 rabbits in each group.Angiogenesis was detected by arteriography on day 28 after treatment and expression of VEGF was detected by immunohistochemical staining on day 30 after treatment.Results There were no differences of collateral vessel count between the EP group,BMSC group and VEGF group.The collateral vessel count in BV group was higher than that of the other three groups.Immunohistochemistry of VEGF showed that the integrated optical density(IOD)in BMSC and VEGF groups increased significantly compared with the EP group; the IOD in BV group was the highest compared with the other three groups.Conclusions Combination bone marrow mesenchymal stem cells and VEGF gene in the treatment of limb ischemia in rabbits can obtain stable and effective expression of VEGF along with significant improvement of limb ischemia.
