Clinical significance of α-galactosidase A activity assay in the screening and diagnosis of Febry disease
10.3760/cma.j.issn.1000-6699.2012.01.017
- VernacularTitle:α-半乳糖苷酶A活性检测对Fabry病筛查和诊断的意义
- Author:
Yina CHANG
;
Xianxia JU
;
Qin CUI
;
Kunlin WANG
;
Hongwen ZHOU
- Publication Type:Journal Article
- Keywords:
Fabry disease;
α-Galactosidase A;
Peripheral blood granulocytes;
Plasma;
Screening
- From:
Chinese Journal of Endocrinology and Metabolism
2012;28(1):54-57
- CountryChina
- Language:Chinese
-
Abstract:
α-Galactosidase A (α-Gal A ) activities in plasma and peripheral blood granulocytes of 100healthy subjects and one patient with Fabry disease was determined by means of fluorogenic substrate.The results showed that the enzymatic activities of peripheral blood granulocytes and plasma in 100 subjects were (51.97 ± 15.24)and(148.08±26.30) nmol · h-1 · ml-1 respectively.The α-Gal A activities in plasma and granulocytes were positively correlated( r=0.533,P＜0.01 ).The enzymatic activities in peripheral blood granulocytes and plasma of the patients with Fabry disease were 1.05 and 10.06 nmol · h-1 · ml-1 respectively,both much lower than those of 100healthy subjects.These results suggest that α-Gal A activity in plasma and peripheral blood granulocytes can be used for diagnosis and screening of Fabry disease.
