Effect of of propofol repeated anesthesia on expression of CaMK Ⅱα in hippocampus in neonatal rats

VernacularTitle:异丙酚重复麻醉对新生大鼠海马CaMKⅡα表达的影响
Author: Shujun PENG ; Ping CHEN ; Jin GAO ; Shengfen TU ; Shiqi XIANG
Publication Type:Journal Article
Keywords: Propofol; Infant,newborn; Calcium-calmodulin-dependent protein kinase type 2; Hippocampus
From: Chinese Journal of Anesthesiology 2011;31(11):1335-1337
CountryChina
Language:Chinese
Abstract: ObjectiveTo investigate the effect of propofol repeated anesthesia on the expression of CaMK Ⅱ α in the hippocampus in neonatal rats.MethodsThirty-two SD rats aged 7 days weighing 12-16 g were randomly divided into 2 groups (n =16 each): group C received intraperitoneal 0.9％ normal saline 7.5 ml/kg once a day for 7 days and group P received propofol 75 mg/kg once a day for 7 days.Learning and memory function were assessed using Morris warier maze at 28 days old of rats.The animals were sacrificed at 24 h after the tests and brain tissues were removed.The expression of CaMK Ⅱ α and phosphorylated CaMK Ⅱ α (pCaMK Ⅱ α) in hippocampal CAI region were determined by immunochemistry and Western bolt.ResultsCompared with group C,the escape latency was significantly prolonged,space exploration time shortened and expression of CaMK Ⅱ α and pCaMK Ⅱ α down-rugulated in group P than in group C( P ＜ 0.01 ).ConclusionPropofol repeated anesthesia decreases congnitive function through down-regulating the expression and inhibiting the activity of CaMK Ⅱ α in hippocampus in neonatal rats.