In vivo study of tyrosine protein kinase Lck inhibited by siRNA in T cells of asthmatic mice
10.3760/cma.j.issn.1008-1372.2011.12.006
- VernacularTitle:SiRNA靶向抑制哮喘小鼠T细胞非受体酪氨酸蛋白激酶Lck的体内研究
- Author:
Shuangyan FANG
;
Caimin SHU
;
Qiongfang YANG
;
Xuefei TAO
;
Yonghua ZHENG
;
Qiaoying. JI
- Publication Type:Journal Article
- Keywords:
RNA,small interfering;
Asthma/TH/ME;
Lymphocyte specific protein tyrosine kinase p56(lck)/ME
- From:
Journal of Chinese Physician
2011;13(12):1603-1606
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveUsing the technology of siRNA to inhibit the gene expression of no-receptor tyrosine protein kinase Lck in T cells of asthmatic mice,and to study the therapeutic effect of Lck specific siRNA in asthmatic mice.MethodsReceptor tyrosine protein kinase Lck specific siRNA fragments were taken from chemosynthesis.In vivo-jetPEITM was used to transfect the siRNA into mice body through tail vein injection.The mice were killed 48 hours later,and the levels of IL-4,IL-17 in bronchoalveolar lavage fluid (BALF) were detected with respondent ELISA kits.The change of inflammatory histopathology in lung was observed with H.E.staining.The expression of Lck in lung was detected with immunohistochemistry (IHC),and the level of Lck in lung tissue homogenate was detected with Western Blot.Results Compared with asthmatic group[ (234.68 ± 11.15 ) pg/ml,( 96.76 ± 8.28 ) pg/ml],the levels of IL-4,IL-17 [ (234.68 ± 11.15)pg/ml,(96.76 ±8.28) pg/ml] in the BALF of siRNA interference group decreased, and the inflammation in the lung relieved.IHC indicated that the expression of Lck in lung decreased and the level of Lck in lung tissue homogenate decreased ( P ＜ 0.05 ).Conclusions Lck specific siRNA could reduce the level of IL-4,IL-17 in the lung tissues of asthmatic mice,and relieve the inflammatory reaction in lung.
