Effect of emulsified isoflurane preconditioning on postsynaptic density protein 95 activation in brain during focal cerebral ischemia-reperfusion injury in rats
10.3760/cma.j.issn.0254-1416.2011.10.022
- VernacularTitle:乳化异氟醚预处理对大鼠局灶性脑缺血再灌注时脑组织突触后致密物质95活化的影响
- Author:
Jiong SHI
;
Zhaoxia DING
;
Zhiping WANG
- Publication Type:Journal Article
- Keywords:
Isoflurane;
Fat emulsions,intravenous;
Membrane proteins;
Reperfusion injury;
Brain;
Ischemic preconditioning
- From:
Chinese Journal of Anesthesiology
2011;31(10):1242-1244
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of emulsified isoflurane preconditioning on postsynaptic density protein 95 (PSD95) activation in brain during focal cerebral ischemia-reperfusion (I/R)injury in rats.Methods Thirty-two pathogen-free male SD rats weighing 280-300 g were randomly divided into 4 groups (n =8each): sham operation group(group S),group I/R,emulsified isoflurane group(group EI) and lipid emulsion group(group LE).Focal cerebral I/R was induced by middle cerebral artery occlusion for 2 h followed by reperfusion.8％ emulsified isoflurane 10.5 ml/mg or 30％ lipid emulsion 10.5 ml/mg was injected intraperitoneally at 24h before I/R in groups EI and LE respectively.The neurologic deficit score (NDS) was evaluated at 6 h of reperfusion and then 4 rats were sacrificed,and brains were removed for determination of phosphorylatied PSD95 (pPSD95) expression in ischemia cortex and hippocampus by Western blot.Four rats were sacrificed at 24 h of reperfusion and then their brains were removed for determination of infarct volume percentage.Results NDS,infarct volume percentage and pPSD95 expression in ischemia cortex and hippocampus were higher in groups I/R,EI and LE than in group S( P ＜ 0.01 ).NDS,infarct volume percentage and pPSD95 expression in ischemia cortex and hippocampus were lower in group EI than in group I/R( P ＜ 0.05).There was no significant difference in NDS,infarct volume percentage and pPSD95 expression between groups I/R and LE(P ＞ 0.05).Conclusion Emulsified isoflurane preconditioning can attenuate focal cerebral I/R injury in rats by inhibiting PSD95 activation in brain.
