Expression of nitric oxide synthase in the Aβ transgenic drosophila

VernacularTitle:一氧化氮合酶在β-淀粉样蛋白转基因果蝇中的表达
Author: Genfa WANG ; Honglian ZHU
Publication Type:Journal Article
Keywords: Nitric oxide synthase/ME; Amyloid beta-protein/GE; Drosophila/ME/GE; Transgenes
From: Journal of Chinese Physician 2011;13(12):1631-1633
CountryChina
Language:Chinese
Abstract: ObjectiveTo investigate the expression and effects of nitric oxide synthase in the Abeta transgenic drosophila.MethodsmRNA was obtained from 20 Abeta transgenic drosophila and 20 wild type drosophila,and cDNA was synthesized by reverse transcription.The expression of nitric oxide synthase mRNA was detected by RT-PCR.ResultsThe expression of nitric oxide synthase mRNA (0.020 ± 0.006)in the Abeta transgenic drosophila was significantly lower than that in the wild type drosophila ( 1.000 ±0.149),there had significant difference between Abeta transgenic drosophila and wild type drosophila ( P ＜ 0.05)..ConclusionsNitric oxide generated by nitric oxide synthase was an important messenger molecule,and it was closely related to Alzheimer's disease.