Effects of dexamethasone administration at different times on intestinal ischemia-reperfusion injury and inducible nitric oxide syntlase activity in mice
10.3760/cma.j.issn.0254-1416.2011.11.029
- VernacularTitle:地塞米松不同时期给药对小鼠肠缺血再灌注损伤及诱导型一氧化氮合酶活性的影响
- Author:
Yanling WANG
;
Wenhua ZHANG
;
Wanling GAO
;
Rui ZHANG
;
Chulian GONG
;
Ziqing HEI
- Publication Type:Journal Article
- Keywords:
Dexamethasone;
Ischemic preconditioning;
Reperfusion injury;
Intestines;
Nitric oxide synthase type Ⅱ
- From:
Chinese Journal of Anesthesiology
2011;31(11):1391-1393
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo evaluate the efffects dexamethasone (DEX) administration at different times on intestinal ischemia-reperfusion(I/R) injury and inducible nitric oxide synthase(iNOS) activity in mice.Methods Tirty-five healthy male Kunming mice weighing 20-24 g were randomly divided into 5 groups( n =7 each): Sham operation group (group Ⅰ ); intestinal I/R group (group Ⅱ ); DEX administration before ischemia group (group Ⅲ ); DEX administration during ischemia group ( group Ⅳ) and DEX administration at the begining of reperfusion group (group Ⅴ ).Intestinal I/R injury was induced by clamping the superior mesenteric artery for 30 min.Normal saline10 mg/kg,DEX 10 mg/kg was injected iv at 30 min before ischemia in groups Ⅱ and Ⅲ respectively.DEX 10 mg/kg was injected iv at 5 min of ischemia in groupⅣ and immediately at the begining of reperfusion in group Ⅴ.The mice were sacrificed at 3 h of reperfusion,and then the small intestinal tissues were taken for determination of intestinal pathological score( Chiu score),iNOS activity and nitric oxide (NO) content.ResultsChiu score was significantly higher in groups Ⅱ - Ⅴ,and iNOS activity and NO content were sinificantly higher in groups Ⅱ,Ⅳ and Ⅴ than in group Ⅰ ( P ＜ 0.05).Chiu score,iNOS activity and NO content were sinificantly lower in group Ⅲ,and were higher in group Ⅴ than in group Ⅱ ( P ＜ 0.05).There was no significant difference in the indexes mentioned above between groups Ⅱ and Ⅳ ( P ＞ 0.05).ConclusionDEX administration before ischemia can reduce intestinal I/R injury by inhibiting iNOS activity; DEX administration during ischemia has no effcet on intestinal I/R injury and iNOS activity; DEX administration at the begining of reperfusion aggravates intestinal I/R injury by enhancing iNOS activity.
