Value of detection of fecal Alu repetitive sequences in the diagnosis of pancreatic cancer

VernacularTitle:粪便Alu序列的检测在胰腺癌诊断中的价值
Author: Yan REN ; Jun GAO ; Xiaowei WANG ; Jianqiang LIU ; Junjun GU ; Jing JIN ; Yanfang GONG ; Zhaoshen LI
Publication Type:Journal Article
Keywords: Pancreatic neoplasms; Alu repeats; Diagnosis; Real-time PCR; Feces
From: Chinese Journal of Pancreatology 2011;11(6):383-385
CountryChina
Language:Chinese
Abstract: ObjectiveTo detect the Alu expression in the stool of patients with pancreatic cancer and investigate its value in the diagnosis of pancreatic cancer.MethodsStool samples were obtained from patients with pancreatic cancer (PC) ( n =41 ),chronic pancreatitis (CP) ( n =27 ) and healthy subjects ( n =23 ),the DNA was extracted from the stool and the expression of Alu repetitive sequences was subjected to quantitative analysis by the real-time PCR.ResultsThe expressions of Alu repetitive sequences in PC,CP,and healthy subjects were (5.17 ± 0.99 ),( 3.79 ± 0.94),(0.28 ± 0.35 ) rig/g,and the difference among the three groups was statistically significant (P ＜0.05).The AUC of PC was 74.8％ with the 95％ CI 0.661 ～0.835,and the sensitivity,specificity was 75.6％ and 67.1％,respectively.ConclusionsAlu repetitive sequences are highly expressed in the stool of patients with pancreatic cancer,and it is of value in the diagnosis of pancreatic cancer.