Role of annexin A1 in human pulmonary arterial smooth muscle cells proliferation induced by hypoxia

VernacularTitle:膜联蛋白A1在低氧诱发人肺动脉平滑肌细胞增殖中的作用
Author: Jing ZENG ; Bin YI ; Zhi WANG ; Bin GUO ; Kaizhi LU ; Xiaobin WANG
Publication Type:Journal Article
Keywords: Annexin A1; Cell hypoxia; Myocytes; Smooth muscle; Pulmonary artery; Cell proliferation
From: Chinese Journal of Anesthesiology 2011;31(10):1239-1241
CountryChina
Language:Chinese
Abstract: Objective To investigate the role of annexin A1 (ANXA1) in human pulmonary arterial smooth muscle cells (PASMCs) proliferation induced by hypoxia.Methods Primary cultured human PASMCs were randomly divided into 5 groups ( n =60 each):normal oxygen group (group N) and hypoxia 2,8,12 and 24 h groups(groups H1-4).Group N was treated with 21％ O2 for 24 h,and groups H1-4 was treated with 3％ O2 for 2,8,12 and 24 h respectively.ANXA1 mRNA and protein expression was determined by RT-PCR and Western blot.Proliferation of PASMCs were determined by MTT and 3 H-TdR methods.Results ANXA1 mRNA and protein expression was down-regulated,proliferation of PASMCs was enhanced in groups H1-4 as compared with group N (P ＜ 0.05).ANXA1 mRNA and protein expression was down-regulated gradually,proliferation of PASMCs was enhanced gradually in groups H1-4 ( P ＜ 0.05 ).Conclusion The expression of ANXA1 is down-regulated in human PASMCs during hypoxia treatment,which might be the mechanism of human PASMCs proliferation induced by hypoxia.