The effect of α-tocopherol on fibrosis of chronic pancreatitis rat
10.3760/cma.j.issn.1674-1935.2011.06.016
- VernacularTitle:α-生育酚对慢性胰腺炎大鼠胰腺纤维化的影响
- Author:
Fei JIANG
;
Xiaohua MAN
;
Yanfang GONG
;
Jun GAO
;
Zhuan LIAO
;
Lianghao HU
;
Zhaoshen LI
- Publication Type:Journal Article
- Keywords:
Pancreatitis chronic;
Oxidative stress;
Fibrsis;
α-Tocopherol
- From:
Chinese Journal of Pancreatology
2011;11(6):430-432
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of α-tocopherol on fibrosis of chronic pancreatitis (CP) rat and explore its mechanism.MethodsMale Wistar rats were randomly divided into control group,acute necrotizing pancreatitis (ANP) group,α-tocopherol group.CP was induced by dibutyltindich loride ( 8 mg/kg) infusion into the tail vein.Gastric lavage of α-tocopherol (800 mg/kg body weight,daily) was started 24 hours after dibutyhindich loride infusion for 4 weeks.The rats in ANP and control group received 0.6 ml salad oil gastric lavage.The rats were sacrificed 4 weeks later.Pancreatic tissue was harvested for histological examination and collagen staining,and measurement of the levels of hydroxyproline and malondialdehyde (MDA) of the pancreas were performed.The mRNA expression of transforming growth factor (TGF)-β１ was measured by real time PCR.ResultsAfter gastric lavage for 4 weeks,the pancreatic tissue inflammation,fiber deposition and abnormal structure in rats of α-tocopherol group were greatly reduced.The levels of MDA and hydroxyproline in rats of α-tocopherol group were significantly lower than those in ANP group [ (0.40 ±0.20) vs (1.07 ±0.41) nmol/100mg,(402.49 ±27.62) vs (664.92 ±29.04) μg/g,P＜0.05].The expressions of TGF-β1 mRNA in rats of o-tocopherol group were significantly lower than those in ANP group (2.24 ± 0.89 vs 3.35 ± 0.66,P ＜ 0.05 ).Conclusions Tocopherol gamma can improve pancreatic inflammation and fibrosis by reducing the oxidative stress level and down-regulating the expression of TGFβ1mRNA in rats with CP.
