Effect of therapeutic hypercapnia on hepatic ischemia-reperfusion injury in rat liver transplantation
10.3760/cma.j.issn.0254-1416.2011.11.022
- VernacularTitle:治疗性高碳酸血症对大鼠移植肝脏缺血再灌注损伤的影响
- Author:
Xiaoguang CUI
;
Lijuan ZHANG
;
Jing TAN
;
Yueping GUO
;
Wenzhi LI
- Publication Type:Journal Article
- Keywords:
Hypercapnia;
Liver transplantation;
Reperfusion injury
- From:
Chinese Journal of Anesthesiology
2011;31(11):1366-1369
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of therapeutic hypercapnia on hepatic ischemia-reperfusion (I/R) injury in rat liver transplantation.MethodsMale specific pathogen-free adult Wistar rats aged 6 weeks weighing 220-280 g were used in this study.Sixteen rats in which liver transplantation was successfully performed were randomly divided into 2 groups ( n =8 each): liver transplantation group (group LT) and therapeutic hypercapnia group (group TH).In group TH,PaCO2 was maintained at 80-100 mm Hg by inhalation of CO2 for 1 h at the begining of reperfusion.MAP,PaO2 and PaCO2 was recorded during reperfusion.Blood samples were obtained at 2 h of reperfusion for determination of serum ALT,AST,TNF-α,IL-1 and IL-6 levels,and then the rats were sacrificed and transplanted liver was immediately removed for determination of NF-κB activity and apoptosis and microscopic examination.The apoptotic index was calculated.ResultsMAP,PaO2 and PaCO2 were higher,and serum ALT,AST,TNF-α,IL-1 and IL-6 levels,NF-κB activity and apoptotic index lower in group TH than in group LT ( P ＜ 0.05).The histopathologic damage was ameliorated in group TH as compared with group LT.Conclusion Therapeutic hypercapnia can attenuate hepatic I/R injury in rat liver transplantation by inhibiting inflammatory response and apoptosis.
