Effects of IL-8 on the tight junction of vascular endothelial cells
10.3760/cma.j.issn.1673-4181.2011.06.004
- VernacularTitle:IL-8对血管内皮细胞紧密连接的影响
- Author:
Yi YU
;
Xiaoheng LIU
;
Yang SHEN
;
Ting GAO
;
Min GAO
;
Yunlong MA
;
Qiang FU
;
Min KANG
;
Jianmin ZHAO
- Publication Type:Journal Article
- Keywords:
Interleukin-8;
Vascular endothelial cell;
Tight junction
- From:
International Journal of Biomedical Engineering
2011;34(6):336-339
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of IL-8 on the tight junction of vascular endothelial cells.Methods Immunofluorescence was used to observe the modality and the distribution of three tight junction proteins (occludin,claudin-5 and ZO-1) of the EA.hy926 cells treated with IL-8 under different concentrations and different times.RT-PCR was used to measure the mRNA expression of these three proteins.Results The results demonstrated that IL-8 could change the distribution of occludin,claudin-5 and ZO-1 in EA.hy926 cells,and the mRNA expression of occludin,claudin-5 and ZO-1 decreased with the increase of IL-8 concentration and treated time.Conclusion The effects of IL-8 on the distribution and the expression of occludin,claudin-5 and ZO-1 are dose and time-dependent.
