Effects of vascular endothelial growth factor from pulmonary stromal cells on differentiation and function of dendritic cells

VernacularTitle:肺脏基质细胞分泌的血管内皮生长因子对树突状细胞分化及功能的影响
Author: Huanxing LIU ; Wenwen WANG
Publication Type:Journal Article
Keywords: Dendritic cells; Vascular endothelial growth factors; Antigens,differentiation; Immune tolerance
From: Chinese Journal of Geriatrics 2011;30(12):1043-1046
CountryChina
Language:Chinese
Abstract: Objective To observe the effects of vascular endothelial growth factor(VEGF)from pulmonary stromal cells on differentiation and function of mature dendritic cells (mDC) and the immune tolerance. Methods Co-culture system of murine pulmonary stroma cells (MPSC)/mDC was established as control group,and VEGF-Ab add-on treatment to co-culture cells as experimental group.Cytokines secreted from pulmonary stroma cells were detected by RT-PCR.The expression of cellular phenotype and ability of the mDC-induced T cells reproductive activity were detected by flow cytometry and CCK-8,respectively.Results CD86 expression of VEGF-Ab group was higher than of control group (P ＜ 0.05).There was no difference in the ability of mDC-induced T cells reproductive activity between the two groups(P＞0.05).Conclusions VEGF might be involved in the regulation of immune tolerance by reducing expression of CD86 in dendritic cells.