Role of peroxisome proliferator-activated receptors gamma related signaling pathway in the development of rat hyperlipidemia
10.3760/cma.j.issn.0254-9026.2011.12.021
- VernacularTitle:过氧化物酶体增殖物激活受体信号通路在大鼠高脂血症发生发展中的作用
- Author:
Fenghua LV
;
Jianzhi GAO
;
Jinying ZHANG
- Publication Type:Journal Article
- Keywords:
Hyperlipidemias;
PPARγ;
Caspase;
Folic acid
- From:
Chinese Journal of Geriatrics
2011;30(12):1047-1050
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the role of peroxisome proliferator-activated receptor gamma (PPARγ)/Caspase-8/Caspase-3 signaling pathway in the development of hyperlipidemia in rats.Methods 60 healthy male SD rats 〔4-week old,weight (110 ± 10) g〕 were randomly divided into control group,high-fat diet group,folic acid group,vitamin B12 group,folic acid and vitamin B12 group.After one week's feeding for adaptability,the groups of foloc acid,vitamine B12 and foloc acid + vitamineB12 were dealt with intraperitoneal injection of folic acid (0.5 mg/d),vitamin B12 (0.05mg/d),and folic acid (0.5 mg/d) plus vitamin B12 (0.05 mg/d),respectively,and fed with high fat diet simultaneously.Control group was dealt with intraperitoneal injection of normal saline (0.5 ml/d)and fed with normal diet.High-fat diet group was only fed with high fat diet.Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the mRNA level of PPARy,Caspase-8 and Caspase-3 in aorta abdominalis dissected at 17 weekends.Results Folic acid alone or jointed with vitamin B12 could effectively increase the level of PPARγ,while decrease the mRNA levels of Caspase8 and Caspase-3 as compared with high-fat diet group (P＜0.05),and folic acid plus vitamin B12 was more effective than folic acid alone (P＜0.05).Conclusions Folic acid alone or joined with vitamin B12 can improve the mRNA levels of PPARγ,Caspase-8 and Caspase-3 in vascular wall to protect endothelial injury from hyperlipidemia.
