Establishment and preliminary evaluation of a rapid detection method for germ tube-specific antigens of Candida albicans
10.3760/cma.j.issn.0412-4030.2011.12.013
- VernacularTitle:白念珠菌芽管特异性抗原快速检测方法的建立和初步评价
- Author:
Guizhi ZHAO
;
Huixia HAN
;
Qiuhe SONG
;
Baiyu ZHONG
;
Hongguang LU
;
Lu WANG
- Publication Type:Journal Article
- Keywords:
Candida albicans;
Antibodies,monoclonal;
Enzyme-linked immunosorbent assay;
Sensitivity and specificity
- From:
Chinese Journal of Dermatology
2011;44(12):861-864
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a double-antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) for the rapid detection of germ tube-specific antigens of Candida albicans,and to evaluate its specificity and sensitivity.Methods A DAS-ELISA was established with the monoclonal antibody McAb03.2C1-C2 as the primary antibody,and horseradish peroxidase (HRP) labeled McAb03.2C1-C2 as the secondary antibody.The established assay was used to detect germ tube-specific antigens of Candida albicans in sera from 5 patients with systemic Candida albicans infection and from 6 rabbit models at 12,24,48,72hours,on week 1,2 after infection with Candida albicans.Results A good liner relationship was observed between the absorbance value at 495 nm and antigen concentrations when the titer of McAb03.2C1-C2 was 1 ∶ 4000 and the concentration of coated antigen varied from 1.25 to 40 μg/ml.The specificity and sensitivity of the DAS-ELISA were 95％ and 92％ respectively in the detection of germ tube-specific antigens in the rabbit models.The results of detection with DAS-ELISA in serum specimens from the patients were consistent with those with the routine method.Conclusions A DAS-ELISA is primarily established for the rapid detection of germ tube-specific antigens of Candida albicans,and has shown a satisfactory sensitivity and specificity in the animal model experiment.
