Simultaneous determination of evodiamine and rutaecarpine of compound Wuzhuyu ointment by HPLC
10.3760/cma.j.issn.1673-4246.2011.12.017
- VernacularTitle:HPLC测定复方吴茱萸软膏中吴茱萸碱和吴茱萸次碱的含量
- Author:
Xuehong ZHOU
;
Zhihao LI
- Publication Type:Journal Article
- Keywords:
Evodiamine;
Rutaecarpine;
Compound Wuzhuyuointment;
HPLC
- From:
International Journal of Traditional Chinese Medicine
2011;33(12):1101-1103
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of evodiamine and rutaecarpine of compound wuzhuyu ointment.Methods HPLC method was used with Agilent HC-C18(250 mm× 4.6 mm,5 μm) column and the mobile phase being acetonitrile-0.08％ ocane sutfonic acid sodium(41:59).The flow rate was 0.8 ml/min,the column temperature was 30℃,and the UV detector was set at 225 nm.Results The linear ranges of evodiamine and rutaecarpine were 0.62～ 12.40 μg/ml (r=0.9997) and 0.24～4.8 0 μg/ml (r=0.9996),respectively.The average recovery of evodiamine and rutaecarpine was 97.9％ and 98.0％,respectively.Conclusion The method is simple,repeatable and accurate.It can be applied in quantitative determination of evodiamine and rutaecarpine in compound Wuzhuyu ointment.
