Construction of lentivirus vector expressing antigene RNA and ferritin gene
10.3760/cma.j.issn.0254-1416.2011.09.019
- VernacularTitle:重组抗基因RNA与铁蛋白基因双启动子慢病毒表达载体的构建
- Author:
Feng GAO
;
Xiaoyan WEN
;
Xijiang LIU
;
Huilian BU
;
Fei CAO
;
Xuebi TIAN
;
Hui YANG
;
Peng WANG
;
Yuke TIAN
- Publication Type:Journal Article
- Keywords:
RNA;
Ferritins;
Lentivirus
- From:
Chinese Journal of Anesthesiology
2011;31(9):1096-1098
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct lentivirus vector expressing antigene RNA and ferritin gene.Methods Intermediate plasmid pGC-FU-HF was constructed by transfecting lentivirus vector pGC-FU with heavy chain ferritin subunit gene.The target plasmid pGC-agRNA-HF was subsequently constructed by transfecting the intermediate plasmid with β-arrestin 2 antigene RNA.The NG108-15 cells were transfected with the target plasmid.The titre of lentivirus vector was measured by RT-PCR.The expression of antigene RNA and ferritin gene was determined by Western blot and RT-PCR.Results Lentivirus vector was successfully transfected with antigene RNA and ferritin gene.The titre of lentivirus vector was 2.00 × 109 TU/ml.The expression of β-arrestin2 protein was down-regulated and the expression of ferritin protein up-regulated in the NG108-15 cells after being transfected with the lentivirus vector.Conclusion Lentivirus vector expressing antigene RNA and ferritin gene has been successfully constructed.
