The effect of LI-cadherin-SiRNA protein on transplanted human hepatoceilular carcinom in nude mice
10.3760/cma.j.issn.1007-631X.2011.12.019
- VernacularTitle:SiRNA干扰LI-cadherin对荷人肝癌裸鼠移植瘤的影响
- Author:
Zhengjun FAN
;
Bo CHENG
;
Shishi QIAO
;
Jianfeng XUE
;
Xiangjie FANG
;
Yanjie LIU
- Publication Type:Journal Article
- Keywords:
RNA,small interfering;
Liver neoplasms,experimental;
Liver intestine cadherin
- From:
Chinese Journal of General Surgery
2011;26(12):1031-1034
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of LI-cadherin- SiRNA protein on the growth and metastatic potentials of transplanted human hepatocellular carcinoma cell lines (Hep3B) in nude mice.Methods We transfected LI-cadherin- SiRNA to Hep3B cells,Hep3B cell suspension (transfected or control ) was injected subcapsullaryly into the spleen of nude mice,hepatic metastasis was observed by naked eye and immunohistochemistry.In addition,Western-blot was used to detect the level of LI-cadherin in different metastatic site.Results (1) Hep3B was green after successful transfect interference vector under fluorescence inverted microscope,in this study,the transfect rate was 80％.(2) Hep3B liver metastasis model in nude mice was established.The metastasis rates in the empty plasmid carrying group,the control group and the SiRNA transfect group were 50％,60％ and 80％,respectively.The number of metastasis caner nodules in the SiRNA transfect group was 26,significantly higher than other two groups.(3) The level of protein expression for LI-cadherin in the SiRNA transfect group is significantly lower than the control group and the empty plasmid carrying group.Conclusions LI-cadherin is crucial and important for the adhension capability of HCC in its migration.SiRNA transfected LI-cadherin increases the metastasis in a nude mouse model inoculated with human hepatocellular carcinoma cell lines.
