Flow cytometry method for antifungal susceptibility test and its clinical application
10.3760/cma.j.issn.1674-2397.2011.06.005
- VernacularTitle:流式细胞术在真菌药物敏感性试验中的临床应用
- Author:
Ruilong XU
;
Yijun ZHU
;
Lihong BO
;
Jianbo XU
;
Yun PAN
;
Xiaoyun SHAN
;
Zhaojing ZHENG
;
Junqing WU
;
Huijiao WANG
- Publication Type:Journal Article
- Keywords:
Flow cytometry;
Fungi;
Microbial sensitivity tests
- From:
Chinese Journal of Clinical Infectious Diseases
2011;04(6):343-347
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a rapid detection method of flow cytometry for antifungal susceptibility testing.Methods The experiment conditions of flow cytometry were optimized and the minimum inhibitory concentrations (M1Cs) of 230 strains of Candida spp to flucytosin,fluconazol,itraconazole,amphotericin B were detected.The MIC results obtained by flow cytometry were compared with M27-A2 reference assay.Results The agreement of MIC results between flow cytometry and M27-A2method was 85.0％ - 100％.Conclusion Flow cytometry can be used as a rapid and reliable method for antifungal susceptibility testing.
