Effects of hydrogen sulfide on hepatic ischemia-reperfusion injury in rats
10.3760/cma.j.issn.0254-1416.2011.09.030
- VernacularTitle:硫化氢对大鼠肝缺血再灌注损伤的影响
- Author:
Jin DU
;
Guolin WANG
- Publication Type:Journal Article
- Keywords:
Hydrogen sulfide;
Reperfusion injury;
Liver
- From:
Chinese Journal of Anesthesiology
2011;31(9):1136-1138
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of hydrogen sulfide on hepatic ischemia-reperfusion injury in rats.Methods Thirty healthy male SD rats weighing 220-250 g were randomly divided into sham operation group (S group),ischemia-reperfusion group(IR group) and different doses of hydrogen sulfide groups (H2S1～3 groups),every group contains six rats.In S group hepatic portal was only exposed but not ligated.In IR group the left and middle lobes of hepatic pedicle,portal vein and hepatic artery were clamped for 1 h and then reperfuse.H2 S1～3groups received intraperitoneal injection of NaHS 14,28,56 μmol/kg at 5 min before reperfusion.Blood samples were collected for measuring the activity of alanine transaminase (ALT) and aspartate transaminase (AST) in serum at 6 h after reperfusion.Liver tissue samples were collected for measurement the glutathione (GSH) content and pathologic examination.Results Compared with S group,the activity of ALT and AST in serum was increased,GSH content in liver tissue decreased in IR group ; Compared with IR group,the activity of ALT and AST in serum was decreased,GSH content in liver tissue increased in H2 S1～3 groups.Hepatic tissue pathologic injury was reduced in H2 S1～3 groups as compared with IR group.Conclusion Hydrogen sulfide can reduce hepatic ischemia-reperfusion injury in rats.
