Ebselen can attenuate the ischemia-reperfusion injury of the rat lungs from non-heart -beating donors
10.3760/cma.j.issn.0254-1785.2011.12.011
- VernacularTitle:依布硒林减轻无心跳大鼠供者供肺的缺血再灌注损伤
- Author:
Dongshan LIAO
;
Kun CHEN
;
Yi YU
;
Qing HUANG
;
Zengqi LI
;
Chongxian LIAO
- Publication Type:Journal Article
- Keywords:
Rats;
Lung transplantation;
Reperfusion injury;
Ebselen
- From:
Chinese Journal of Organ Transplantation
2011;32(12):746-748
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of Ebselen on the ischemia-reperfusion injury (IRI) in rat lungs from non-heart-beating donors (NHBD).Methods Forty Sprague-Dawley rats were paired randomly divided into two groups:group Ⅰ,NHBD with 30 min of warm ischemia time (WIT); group Ⅱ,NHBD with 30 min of WIT and administration of Ebselen.The donor lungs remained ventilated at the room temperature for 30 min after asystolia and then flushed with LPD solution.The recipient rats underwent left lung transplantation.The recipients of group Ⅱ were administered with Ebselen (500 mg/kg body weight) one h before transplantation.Results All the recipients survived during the observation period.In the group Ⅱ,the MDA of the pulmonary tissue was 0.631 ± 0.23 nrmol/mg protein,and the polymorphonuclear neutrophils and the total protein of the bronchoalveolar lavage fluid were (78.4 ± 35.2) × 107/L and (0.41 ± 0.12) mg/ml respectively.The MPO was (25.09 ± 1.19) ％ and W/D was 0.359 ± 0.017.There was significant difference between group Ⅱ and group Ⅰ (all P＜ 0.05).Conclusion The administration of ebselen is an effective treatment to attenuate the acute injury resulted from the ischermia-reperfusion in the rat lungs from non-heart-beating donors.
