Strategies for novel protein biomarker identification through multiple reaction monitoring mass spectrometry
10.3760/cma.j.issn.1009-9158.2011.06.002
- VernacularTitle:基于MRM-MS技术的蛋白质生物标志物研究的前景
- Author:
Bing HAN
;
Lishun WANG
- Publication Type:Journal Article
- Keywords:
Biological markers;
Proteome;
Peptides;
Mass spectrometry
- From:
Chinese Journal of Laboratory Medicine
2011;34(6):484-488
- CountryChina
- Language:Chinese
-
Abstract:
With the development of proteomic technologies, large numbers of putative biomarkers have been screened out but few of them are put into the clinical application.Recently, MRM-MS based approaches and strategies have aroused extensive concern in domestic and overseas clinical laboratory community because of their cost-efficiency, time-saving, high analytical sensitivity and accuracy.MRM-MS based approaches, especially SISCAPA, may gradually become a key technology for the protein biomarker research in the near future.In this review, the bottleneck existing in the field of protein biomarkers research, and features as well as applications of MRM-MS are discussed and the future trend of research is prospected.
