Expression of bradykinin as a substrate of CD26/DPP Ⅳ in rats ischemia/reperfusion injury following lung transplantation
10.3760/cma.j.issn.0254-1785.2011.07.013
- VernacularTitle:CD26/二肽酰肽酶Ⅳ底物缓激肽在大鼠肺移植缺血再灌注损伤中的表达
- Author:
Zheng TANG
;
Jianjun WANG
;
Wei ZHAI
;
Zuo CHEN
- Publication Type:Journal Article
- Keywords:
Lung transplantation;
Reperfusion injury;
CD26/DDP Ⅳ;
Bradykinin;
Proteome
- From:
Chinese Journal of Organ Transplantation
2011;32(7):426-429
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of bradykinin as a substrate of CD26/DPP Ⅳ in rats with ischemia/reperfusion injury following lung transplantation (LTx). Methods Thirty-six syngeneic male SD rats were randomly allocated into control group and experimental group (n= 18each), and 36 rats served as donors. In control group and experimental group, the lungs were flushperfused and preserved with LPD and LPD+ CD26/DPP TV catalytic inhibitor (AB192) respectively,and LTx was performed 18 h after cold ischemia Histopathological studies were also done on the same locus of lung specimens and staining for bradykinin. The different proteins were separated by means of immobilized PH gradient-based two-dimensional gel electrophoresis. The differential expression of bradykinin was compared by Western blotting. Results The expression of bradykinin in control group was significantly higher than in experimental group. As compared with experimental group,bradykinin expression was significantly up-regulated in control group (P <0 . 05 ). Conclusion Inhibitor of CD26/DDP Ⅳ enzymatic activity significantly ameliorated ischemia/reperfusion injury in the early stage after LTx, which is probably associated with inhibition of the protein expression of bradykinin.
