Cotransplanation of parathyroid cell cultured under simulated microgravity condition and allogeneic testicular sertoli cell
10.3760/cma.j.issn.0254-1785.2011.06.012
- VernacularTitle:模拟微重力下三维培养的大鼠甲状旁腺细胞与睾丸塞尔托利细胞联合移植
- Author:
Shuangfeng XUE
;
Dajiang ZHAO
;
Xiuqing DUAN
- Publication Type:Journal Article
- Keywords:
Weighlessness simulation;
3-dimension culture;
Parathyroid;
Sertoli cells
- From:
Chinese Journal of Organ Transplantation
2011;32(6):367-371
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the function and survival of parathyroid cells cultured under simulated microgravity condition after cotransplanation of syngeneic allogeneic testicular sertoli cells.Methods Parathyroid cells in SD rats were assigned to flask-culture or bioreactor-culture.Allogeneic testicular sertoli cells in SD rats were cultured by using routine method.The recipients of hypoparathyroidism Wistar rat models were divided into 3 groups randomly:group A,receiving parathyroid cells(cultured with routine method)transplantation only;group B,receiving parathyroid cells and allogeneic testicular sertoli cells(cultured with routine method)transplantation;group C,receiving parathyroid cells(cultured under simulated microgravity condition)and allogeneic testicular sertoli cells transplantation.Allograft survival,change in cell components,apoptosis of infiltrative lymphocytes and parathyroid cells function were analyzed after transplantation respectively.Results The average survival time in group A,B and C was(17.3±1.6),(43.2±2.4)and (52.5±1.5)days,respectively.There was significant difference among group (P<0.05).In group C,parathyroid cells with strong secreting function grew into scaffold materials and adhered to them.FasL-expressing testicular cells and apoptotic lymphocytes were quite evident between allograft and kidney parenchyma.Conclusion Parathyroid cell cultured under simulated microgravity condition enhances its survival and function after cotransplanation of allogeneic testicular sertoli cell with immune privilege.
