Role of OX40 in mechanisms of memory T cells in islet transplant tolerance
10.3760/cma.j.issn.0254-1785.2011.05.013
- VernacularTitle:抑制记忆性T淋巴细胞共刺激分子OX40延长小鼠胰岛移植物的存活时间
- Author:
Ming CHEN
;
Shuqiu CHEN
;
Xiangnong HU
;
Xiaowen ZHANG
;
Xianchang LI
- Publication Type:Journal Article
- Keywords:
Mice;
Islets of langerhans transplantation;
Memory T cell;
OX40;
Tolerance
- From:
Chinese Journal of Organ Transplantation
2011;32(5):305-308
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of OX40 in the mechanisms of memory T cells in islet transplant tolerance.Methods The expression of OX40 on native, like memory and memory CD8+T cells was detected by RT-PCR. Splenic T cells from B6 mice were injected into Rag-/- mice via the tail vein, and the Rag-/- mice were divided into three groups (n=8 each): control group, given IgG; treatment group, given anti-OX40L; and OX40 knock-out group, given T cells from OX40 knock-out B6 mice spleen. All recipients were induced into diabetes mellitus model after adoptive transfer. Islet transplantation was performed on all Rag-/- mice as recipients. The mean survival time of islet was observed.Results The expression of OX40 in native T cells, like memory T cells and memory T cells was 2.87, 111.24 and 146.15 respectively. The expression of OX40 in like memory and memory T cells was higher than in native T cells (P<0.05). Comparison with control group , The mean survival time of the DBA/2 islet allografts in treatment group (130 days) and OX40 knock-out group (125 days) was significantly longer than in control group (21 days, P<0.05).Conclusion The OX40 expression is high in memory T cells. The mean survival time of the islet allografts can be prolonged by blocking OX40/OX40L pathway. OX40/OX40L pathway may be the key point of transplant tolerance.
