Epithelial-mesenchymal transition of renal tubular epithelial cells under anoxia
10.3760/cma.j.issn.0254-1785.2011.06.011
- VernacularTitle:缺氧条件下大鼠肾小管上皮细胞的离体培养及相关检测
- Author:
Guangheng LUO
;
Li YANG
;
Youping LI
;
Yiping LU
- Publication Type:Journal Article
- Keywords:
Rats;
Epithelial cells;
Anoxia;
Interstitial fibrosis
- From:
Chinese Journal of Organ Transplantation
2011;32(6):363-366
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore whether anoxia can induce expression changes in connective tissue growth factor(CTGF)in renal tubular epithelial cells(TECs)and epithelial-mesenchymal transition of TECs.Methods Rat renal TECs(NRK-52E)anoxia models were established.NRK-52E cells were exposed to anoxia for 4 h.The real-time RT-PCR,Western blotting,immunohistochemical staining were used to detect the expression of CTGF at 6,12,24,48,and 72 h in NRK-52E cells.Morphological changes and cytoskeleton remodeling in NRK-52E cells under anoxia were examined by a laser confocal microscope and BODIPYFL staining respectively.Results Under anoxia,NRK-52E cells became round,enlarged and cytoskeleton was remodeled.The expression levels of CTGF mRNA and protein were up-regulated at 6 h,reached their peak at 48 h:the expression of CTGF mRNA protein was 29.33±0.21 and 1.30±0.02 respectively.Under anoxia,NRK-52E cells underwent an epithelial-mesenchymal transition process,including cytoskeleton remodeling,and morphological changes.Conclusion Anoxia can change the expression of CTGF and other fibrosis-associated genes in NRK-52E cells,and CTGF played an important role in fibrosis process and epithelial-mesenchymal transition development in NRK-52E cells.
