The effect of 3-MA on proliferation, apoptosis and cell cycle of rat endothelial progenitor cells
10.3760/cma.j.issn.1007-631X.2011.07.010
- VernacularTitle:3-甲基腺嘌呤对大鼠内皮祖细胞增殖、凋亡及细胞周期的影响
- Author:
Renda ZHU
;
Xiaoqiang LI
;
Qingyou MENG
;
Hui LIU
;
Fengrui LEI
;
Yeqing ZHANG
- Publication Type:Journal Article
- Keywords:
Stem cells;
Cell proliferation;
Apoptosis;
Cell cycle;
3-methlyadenine
- From:
Chinese Journal of General Surgery
2011;26(7):562-565
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of proliferation,apoptosis and cell cycle of 3-MA on rat endothelial progenitor cells. Methods Bone marrow-derived mononuclear cells were isolated from rat bone marrow by ficoll. There were five groups. The control group and four 3-MA concentration groups: 1. 25 mmol/L,2. 5 mmol/L,5 mmol/L, 10 mmol/L. MTT was used to measure the proliferation of endothelial progenitor cells. Flow cytometry ( FCM) was used to detect cell apoptosis and cell cycle. Results (1)5 mmol/L 3-MA promotes proliferation of endothelial progenitor cells, while 10 mmol/L 3-MA inhibits the proliferation of endothelial progenitor cells (P ＜ 0. 05). (2) 10 mmol/L 3-MA promotes apoptosis of endothelial progenitor cells, compared with the control, the difference was significant ( P ＜ 0. 05 ). (3) 3-MA at the concentration of 5 mmol/L reduces cells at G0/G1 phase and increases S and G2/M phase cells; 10 mmol/L 3-MA induces endothelial progenitor cells blockade at S phase, G2/M phase cells decreased, compared with the control, the difference was significant (P ＜ 0. 05). Conclusions 5 mmol/L 3-MA promotes the proliferation of endothelial progenitor cells. 10 mmol/L 3-MA inhibits the proliferation and promotes apoptosis of endothelial progenitor cells.
