The changes of serum 6-k-PGF1α and TXB2 levels in dog's blood vessel prostheses seeded with CD34+ stem cells
10.3760/cma.j.issn.1007-631X.2011.05.020
- VernacularTitle:骨髓CD34+干细胞种植人工血管后血清中6-k-PGF1α和TXB2的变化与人工血管通畅率的关系
- Author:
Kun WANG
;
Zhenhai YU
;
Guangxin LI
;
Shuguang ZHANG
;
Shiyong QIN
;
Minghai WANG
- Publication Type:Journal Article
- Keywords:
Stem cell transplantation;
Blood vessel prosthesis;
Antigens,CD34;
Prostacyclin;
Thromboxane B2
- From:
Chinese Journal of General Surgery
2011;26(5):421-425
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the 6-Keto-PGF1α,TXB2 and the endotheliosis and thrombosis of the new endomembrance on the prostheses seeded by CD34 stem cells.Method Twelve dogs were evenly divided into two groups; ePTFE prostheses and weaved Dacron prostheses implanted into the abdominal aorta(AA) and inferior vena cava (IVC).in each group, 4 dogs were implanted by prostheses seeded by CD34+ stem cells, 2 dogs were implanted prostheses treated by autogenous blood only as control.The peripheral blood was collected in preoperative and 3 ,7,14,30,60 days postoperation.light and electron microscopy were applied to observe the endotheliosis and thrombosis of the new endomembrance when harvesting all the prostheses in the 60 days.Result The platelet, 6-Keto-PG F1α,TXB2 and P/T in experimental group was significantly different compared with control group.The new endomembrane in control group was significantly thickener than the experimental group.Conclusions CD34+ stem cell inhibits PGF1α and P/T ratio decrease, restrains TXB2 and platelet excessively rise, prevents intimal hyperplasia and thrombosis in a CD34+ stem cells seeded blood vessel prostheses.
