Reversal of multidrug resistance in human colon carcinoma LOVO/5-Fu cells by tetrandrine
10.3760/cma.j.issn.1007-631X.2011.05.003
- VernacularTitle:粉防己碱逆转大肠癌耐药细胞株LOVO/5-Fu多药耐药性的实验研究
- Author:
Kailei WANG
;
Leping LI
;
Changqing JING
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Drug resistance,neoplasm;
Apoptosis;
P-glycoprotein
- From:
Chinese Journal of General Surgery
2011;26(5):367-370
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the reversal effect on MDR1 gene-mediated multidrug resistance in human colon carcinoma LOVO/5-Fu cells by tetrandrine ( Tet) and to clarify its molecular mechanism.Methods LOVO/5-Fu cells were treated for 48 h with Tet.Drug sensitivity was measured by MTT.The cell cycle, apoptosis of cells and expression of P-glycoprotein (P-gp) were determined by flow cytometry assay.Expression of MDR1 mRNA was detected by real-time quantitative PCR (real-time PCR).P-gp expression was detected by Western blot.Results After LOVO/5-Fu cells were treated for 48 h with Tet, the IC50 of 5-Fu decreased to ( 4.15 ± 0.31 ) μg/ml ( P ＜ 0.05 ) ; and the apoptotic rate increased to (3.44% ± 0.28% ) ( P ＜ 0.05) ; the expression of MDR1 mRNA reduced to (570 ± 85) (P ＜ 0.05 ).Conclusions Tetrandrine reverses MDR1 gene-mediated multidrug resistance in human colon carcinoma LOVO/5-Fu cells possibly by inhibiting the expression of MDR1, decreasing the expression of P-gp, thus enhancing the sensitivity of LOVO/5-Fu cells to 5-fluorouracil.
