Oncolytic adenovirus expressing lipocalin 2 suppresses the growth of transplantation tumors of colonic carcinoma
10.3760/cma.j.issn.1007-631X.2011.05.002
- VernacularTitle:携带lipocalin2基因溶瘤腺病毒治疗结肠癌移植瘤的实验研究
- Author:
Jinfeng FENG
;
Bin XU
;
Haiyan GE
;
Xinyuan LIU
- Publication Type:Journal Article
- Keywords:
Colonic neoplasms;
Disease model,animal;
Adenoviruses,human;
Apoptosis;
Lipocalin 2
- From:
Chinese Journal of General Surgery
2011;26(5):363-366
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antitumor activity of the oncolytic adenovirus expressing lipocalin 2 gene for colorectal cancer in vivo.Methods BALB/C nude mice subcutaneously inoculated by SW620 cells and grown tumors were treated with injection of ZD-55 virus, Ad-lipocalin 2 virus and ZD55- lipocalin 2 virus respectively.The weight of implanted tumors and the tumor inhibition rate were calculated to evaluate the anti-tumor effect.Cell apoptosis was determined by TUNEL and the protein expression of VEGF and MVD were determined with immunohistochemistry.Results ZD55-lipocalin 2 inhibited the growth of transplanted tumor more significantly than ZD-55 virus and Ad-lipocalin 2 virus ( P ＜ 0.05 ).Tumor cell apoptosis was upregulated and the MVD reduced significantly in ZD55-lipocalin 2 group in contrast to the other two groups (P ＜0.05).Conclusions ZD55-lipocalin 2 induces apoptosis of colorectal tumor cells and inhibits tumor microvascular formation, slowing down the growth of transplantation tumors.
