Differential proteomic analysis of fluconazole-sensitive Candida albicans before and after tetrandrine treatment
10.3760/cma.j.issn.0412-4030.2011.08.010
- VernacularTitle:白念珠菌经汉防己甲素作用前后的差异蛋白质组学分析
- Author:
Laiqiang GAO
;
Hong ZHANG
;
Xiaoli ZHANG
;
Hui GUO
;
Zhiyuan WANG
;
Xifu WU
;
Gehua ZHANG
- Publication Type:Journal Article
- Keywords:
Candida albicans;
Drug resistance,fungal;
TETRANDRINE;
Proteomics
- From:
Chinese Journal of Dermatology
2011;44(8):560-563
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of tetrandrine on the protein expression profile of C. albicans,and to screen for proteins associated with the effect of tetrandrine. Methods Fluconazole-sensitive C. albicans CA-3 was cultured with or without tetrandrine (250 mg/L) for 6 hours followed by the collection of Candida cells. Total proteins were extracted from these cells and separated by using immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis. Protein spots were detected and analyzed by Image Master 2D Platinum software, and MALDI-TOF/TOF-MS-based method was used to identify these proteins. Results The two-dimensional gel electrophoresis maps of C. albicans proteins before and after the treatment with tetrandrine were successfully obtained with high repeatability. Image analysis revealed a total of 26 differentially expressed protein spots in C. albicans treated with tetrandrine, and 7 differentially expressed proteins were identified by the mass chromatographic analysis, including 1 up-regulated protein (Pst1), and 6 down-regulated proteins (Idh1,Asc1, Rps5, Asn1, Asn1, Srb1 ). Conclusions The expressions of some proteins in fluconazole-sensitive C. albicans experience significant changes after tetrandrine treatment, and Pst1, Idh1, Asc1, Rps5, Asn1, Asn1 and Srb1 may considerab1y contribute to the effects of tetrandrine on C. albicans.
