Effects of tacrolimus on the expression of stem cell factor(SCF)mRNA in human HaCaT keratinocytes and c-kit mRNA in murine B16 melanoma cells
10.3760/cma.j.issn.0412-4030.2011.07.017
- VernacularTitle:他克莫司对HaCaT细胞干细胞因子mRNA及小鼠B16黑素瘤细胞c-kit mRNA表达的影响
- Author:
Junling ZHANG
;
Danfeng SUO
- Publication Type:Journal Article
- From:
Chinese Journal of Dermatology
2011;44(7):509-510
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of tacrolimus on the expression of SCF mRNA in keratinocytes and c-kit mRNA in melanoma cells.Methods Tacrolimus was used to treat cultured HaCaT keratinocytes and B16 melanoma cells for 48 hours.Subsequently,real-time fluorescence quantitative PCR was performed to determine the mRNA expression of SCF in HaCaT cells and of c-kit in B16 cells.Results A significant change was observed in the expression of SCF mRNA in HaCaT cells and c-kit mRNA in B16 cells treated with tacrolimus compared with untreated HaCaT cells and B16 cells,respectively (both P<0.05).Conclusion Tacrolimus can upregulate SCF mRNA expression in HaCaT cells and c-kit mRNA expression in R16 cells.
