Screening for Kaposi sarcoma-associated genes by using Genechip technology
10.3760/cma.j.issn.0412-4030.2011.06.008
- VernacularTitle:基因芯片表达谱筛选Kaposi肉瘤相关基因
- Author:
Hui WANG
;
Guodong LV
;
Xiaodong WANG
;
Yan HUI
;
Hui LIU
;
Renyong LIN
;
Xing WANG
- Publication Type:Journal Article
- Keywords:
Sarcoma,Kaposi;
Oligonucleotide array sequence analysis;
Gene expression profiling;
Differential expression gene
- From:
Chinese Journal of Dermatology
2011;44(6):403-406
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen for Kaposi sarcoma (KS)-related genes. Methods Tissue samples were obtained from the lesion and normal skin of a patient with KS in Xinjiang Uygur Autonomous Region,and total RNA was extracted from these samples and reverse transcribed into cDNA. Real-time fluorescent quantitative reverse transcription PCR (RT-qPCR) was performed to determine the expression of K8.1, K2 and ORF50 in these samples. The cDNA was labeled with fluorescein and hybridized to a human 35K genome array containing 25 100 genes. Subsequently, the signal images were scanned by a laser scanner and acquired images were analyzed by software. Results RT-qPCR revealed the mRNA expression of K8.1, K2 and ORF50 in the KS tissues but not in the normal skin tissues, indicating that there was no crossed contamination in these specimens. Among the 25 100 genes, 1313 genes were identified to be differentially expressed between KS and normal skin tissues, including 756 up-regulated genes and 557 down-regulated genes. These differentially expressed genes, such as myeloid cell leukemia-1 gene (MCI-1), annexins (ANX) and serine proteinase inhibitor Kazal type 5 (SPINK5), were associated with apoptosis, angiogenesis, cell signaling, protein processing, cell cycle regulation, and so on. Conclusion The differentially expressed genes such as MCI-1 and SPINK5 may be associated with the development of KS.
