Influence of the expression of parathyroid hormone 1 receptor to INS-1 cell function under high glucose condition
10.3760/cma.j.issn.1000-6699.2011.06.017
- VernacularTitle:甲状旁腺素受体1表达对高糖状态下INS-1细胞功能影响研究
- Author:
Huasheng LIANG
;
Yuhua ZHONG
;
Yaoming XUE
- Publication Type:Journal Article
- Keywords:
Parathyroid hormone receptor 1;
INS-1 cells;
Insulin synthesis;
Insulin secretion;
High concentration of glucose
- From:
Chinese Journal of Endocrinology and Metabolism
2011;27(6):509-512
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe insulin synthesis and secretion in INS-1 under high glucose, and to clarify the effect of PTH1R. Methods After successful construction of recombinant PTH1R-siRNA vectors in INS-1 cell, insulin secretion and intracellular insulin content of control group, siPTH1R-Negative control group, PTHrP group, and siPTH1R group under 25 mmol/L glucose were measured by radioimmunoassay in INS-1 cell. Intracellular calcium were detected by Fluo-3/AM and the capability of glucose transport was calculated by assaying the uptake of [3H]-2-deoxy-D-glucose in cells.Results Compared with control group, and siPTH1R-NC group, PTHrP group showed increased capability of insulin secretion; PTHrP group had higher intracellular insulin levels than others; PTHrP group showed increased intracellular calcium; the uptake of [3H]-2-deoxy-D-glucose under high glucose after 48h of PTHrP group was increased(all P＜0.01). Conclusion There is a close relationship between PTH1R activation and insulin secretion and synthesis, PTH1R activation may be one of the protective mechanisms in maintaining function of β-cell under high glucose.
