Effects of sevoflurane anesthesia on expression of c-Jun N-terminal kinase and neuronal apoptosis in hippocampus in juvenile rats
10.3760/cma.j.issn.0254-1416.2011.05.013
- VernacularTitle:七氟醚麻醉对幼鼠海马c-Jun氨基末端激酶表达和神经细胞凋亡的影响
- Author:
Long CHEN
;
Mingzhang ZUO
;
Gongpan LIU
;
Yanxi CHEN
;
Yon ZHANG
;
Qin CHENG
;
Maoyin ZHANG
- Publication Type:Journal Article
- Keywords:
Anesthetic;
inhalation;
JNK mitogen-activated protein kinases;
Apoptosis;
Hippocampus;
Neurons
- From:
Chinese Journal of Anesthesiology
2011;31(5):563-565
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of sevoflurane anesthesia on the expression of c-Jun N-terminal kinase (JNK) and neuronal apoptosis in hippocampus in juvenile rats.Methods Forty healthy male SD rats, aged 30-35 days, weighing 100-110 g, were randomly divided into 2 groups (n = 20 each): control group (group C) and sevoflurane group (group S) . Group C inhaled a gas mixture of oxygen and air for 5 h and group S 3% sevoflurane for 5 h. The concentration of oxygen in both groups was maintained at 30% . Ten rats in each group were scarified at 1 h after regaining consciousness and the hippocampi removed for determination of phospho-JNK expression (by immuno-histochemistry and Western blot) and neuronal apoptosis (by TUNEL) . Another 10 rats were selected at 24 h after regaining consciousness to assess the cognitive function using Morris water maze. Results Compared with group C, phospho-JNK expression was significantly up-regulated, the number of apoptotic neurons increased, the latency prolonged and the duration of staying at the original platform quadrant shortened in group C ( P ＜ 0.05 or 0.01) . Conclusion Inhalation of 3.0% sevoflurane can induce neuronal apoptosis in hippocampus by activating JNK signaling pathway, thus leading to cognitive decline in juvenile rats.
