Effects of electroacupuncture of different intensities on cerebral energy metabolism in a rat model of global cerebral ischemia-reperfusion
10.3760/cma.j.issn.0254-1416.2011.04.033
- VernacularTitle:不同强度电针刺激对脑缺血再灌注大鼠脑能量代谢的影响
- Author:
Weiqian TIAN
;
Suyang CUI
;
Fengzhen YAO
;
Baogui LI
- Publication Type:Journal Article
- Keywords:
Electroacupuncture;
Reperfusion injury;
Energy metabolism;
Brain
- From:
Chinese Journal of Anesthesiology
2011;31(4):497-499
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of electroacupuncture (EA) of different intensities on cerebral energy metabolism in a rat model of global cerebral ischemia-reperfusion (I/R) . Methods Forty male SD rats weighing 200-230 g were randomized into 5 groups ( n = 8 each) : group A sham operation; group B global cerebral I/R and C, D, E groups cerebral I/R+ 5, 3, 1 mA EA. Global cerebral I/R was induced by 4-vessel occlusion technique. Bilateral vertebral arteries were permanently occluded by cauterization.Bilateral common carotid arteries were clamped. When the bilateral pupils were completely dilated, the arteries were unclamped. Baihui,Mingmen and Zusanli were electrically stimulated with 5,3,1 mA (30-50 Hz) for 20 min at 1 h of reperfusion in C, D, E groups. The EA was repeated at 12 h of reperfusion. The animals were sacrificed at 24 h of reperfusion.The activities of Na+ -K+ -ATPase, succinodehydrogenase (SDH) and lactic dehydrogenase(LDH) in brain tissue were measured.Results Cerebral I/R significantly increased LDH activity and decreased Na+ -K+ -ATPase activity in group B as compared with group A. EA with 5 mA significantly decreased LDH activity and increased SDH and Na+ -K+ -ATPase activities in group C compared with group B. Conclusion EA can improve the cerebral energy metabolism in a rat model of global cerebral I/R and it is related to the intensity, which may be the mechanism by which EA reduces the global cerebral I/R injury.
