The role of microglial activation in spinal cord in a rat model of persistent postoperative pain evoked by skin/muscle incision and retraction
10.3760/cma.j.issn.0254-1416.2011.04.015
- VernacularTitle:脊髓小胶质细胞活化在大鼠术后持续性痛中的作用
- Author:
Xianhe YIN
;
Xingguo HU
;
Yinming ZENG
- Publication Type:Journal Article
- Keywords:
Mictoglia;
Pain,postoperative;
Spinal cord
- From:
Chinese Journal of Anesthesiology
2011;31(4):442-445
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of microglial activation in spinal cord in a rat model of persistent postoperative pain evoked by skin/muscle incision and retraction (SMIR) .Methods Seventy-two male SD rats weighing 200-250 g in which intrathecal (IT) catheter was successfully inserted were randomly divided into 3 groups ( n = 24 each) : group sham operation; group SMIR and group SMIR + FT minocycline (a specific microglia inhibitor) . The rat model of persistent postoperative pain evoked by SMIR was established according to the method described by Flatters. Pain behavior was assessed by paw mechanical withdrawal threshold ( MWT) to von Frey filament stimulation at 1 day before (T0,baseline) and 3, 7, 12, 22 and 32 days after operation (T1-5,) . Four animals were sacrificed at each time point in each group for detection of the expression of Iba-1 (a specific marker of microglia) in the spinal dorsal horn by immunofluorescence and the microglia was counted. Results MWT was significantly decreasedat T1-4, while the expression of Iba-1 and microglia counts in the spinal dorsal horn were significantly increased at T1, 2 by SMIR in group Ⅱ. IT minocycline significantly attenuated the hyperalgesia induced by SMIR at T1-4 and decreased Iba-1 expression and microglia counts at T1,2 in group Ⅲ. Conclusion Microglial activation in the spinal cord plays an important role in the development and maintenance of SMIR-evoked persistent postoperative pain in rats.
