Effect of sevoflurane pretreatment on renal ischemia-reperfusion-induced apoptosis in kidney in rats
10.3760/cma.j.issn.0254-1416.2011.03.026
- VernacularTitle:七氟醚预先给药对大鼠肾脏缺血再灌注时细胞凋亡的影响
- Author:
Jing WANG
;
Jianbo YU
;
Lirong GONG
;
Haidong LI
;
Man WANG
;
Yue ZHANG
;
Fen ZHOU
;
Jin XU
- Publication Type:Journal Article
- Keywords:
Reperfusion injury;
Kidney;
Apoptosis;
Sevoflurane
- From:
Chinese Journal of Anesthesiology
2011;31(3):360-363
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of sevoflurane pretreatment on renal ischemia-reperfusion (I/R)-induced apoptosis in kidney in rats. Methods Thirty pathogen-free male SD rats weighing 220-260 g were randomized into 3 groups (n=10 each):group control (group C);group I/R and group sevoflurane(group S). Renal I/R was induced by clamping the left renal pedicle for 45 min in I/R and S groups. In group S inhalation of 2.2% sevoflurane in O2 was started at 30 min before operation and maintained throughout the experiment.Venous blood samples were taken at 3 h of reperfusion for determination of serum BUN and Cr concentrations. The animals were then sacrificed and the left kidneys were removed for microscopic examination, detection of apoptosis(by TUNEL)and determination of heme oxygenase-1(HO-1) mRNA and protein expression (by RT-PCR and Western blot).Results Renal I/R significantly increased serum BUN and Cr concentrations, apoptotic index(percentage of apoptotic cells) and the severity of necrosis of renal proximal convoluted tubules (0=normal,4=necrosis of whole segment of proximal convoluted tubules).Sevoflurane inhalation attenuated the I/R-induced changes mentioned above.HO-1 mRNA and protein expression was up-regulated by I/R and HO-1 mRNA expression was further up-regulated by sevoflurane inhalation.Conclusion Sevoflurane pretreatment can protect kidney against I/R injury by attenuating cell apoptosis.Up-regulation of HO-1 mRNA expression may be involved in the mechanism.
