Effect of irrigation with hypothermic artificial cerebral spinal fluid on aquaporin-4 expression in spinal cord following spinal ischemia-reperfusion in rabbits
10.3760/cma.j.issn.0254-1416.2011.03.023
- VernacularTitle:低温人工脑脊液灌流对兔脊髓缺血再灌注时水通道蛋白-4表达的影响
- Author:
Yuying TANG
;
Yanjun LIN
;
Jiman LI
;
Junfeng WAN
;
Wei HUANG
- Publication Type:Journal Article
- Keywords:
Hypothermia,induced;
Cerebrospinal fluid;
Reperfusion injury;
Spinal cord;
Aqua-porin 4
- From:
Chinese Journal of Anesthesiology
2011;31(3):350-353
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of irrigation with hypothermic artificial cerebral-spinal fluid (aCSF)on expression of aquaporin-4(AQP-4) in the spinal cord following spinal ischemia-reperfusion (I/R) in rabbits.Methods Fifty-four adult male New Zealand white rabbits weighing 2.0-2.5 kg were randomly divided into 3 groups (n=18 each):group sham operation(S group); group I/R and group irrigation with hypothermic aCSF (FI group). Spinal I/R was induced by clamping the abdominal aorta below renal artery for 60 min. Hypothermic aCSF(25 X.)was infused at L4,5 interspace at a rate of 30 ml/h and drained from L7,8 interspace during spinal ischemia.Neurological function was evaluated at 4, 24, 48 and 72 h of reperfusion and scored (0=no hind limb activity, 4=hind limb function completely recovered) in 6 animals in each group. Six animals were sacrificed at 4, 24 and 72 h respectively in each group.The lumbar segment (L5-8) was removed for measurement of water content and AQP-4 protein expression (by immuno-histochemistry).Results Neurological function scores were significantly lower,water content was higher and AQP-4 expression smaller in group I/R than in group S. I/R-induced effects were significantly attenuated by irrigation of hypothermic aCSF. Conclusion Irrigation with hypothermic aCSF can ameliorate the spinal cord I/R injuries by up-regulation of AQP-4 expression.
